کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5905730 | 1159926 | 2014 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Bioinformatic identification and experimental validation of miRNAs from foxtail millet (Setaria italica)
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کلمات کلیدی
qRT-PCRMinimal folding free energy indexMFEIDCL1GSSMFEWGSAdenosine - آدنوزینBLAST, basic local alignment search tool - ابزار پایهای برای جستجوی برهم نهیهای موضعی، بلاستFoxtail millet - ارزن FoxtailEST - استBlast - انفجارTargets - اهدافUracil - اوراسیل، یوراسیلwhole-genome shotgun - تفنگ کامل ژنومExpressed Sequence Tag - تکرار اظهار نظرRISC - خطرRNA-Induced Silencing Complex - مجتمع خاموش کننده RNA inducedMicroRNA - میکرو RNA Nucleotide - نوکلئوتیدGene ontology - هستیشناسی ژنیquantitative real-time PCR - واکنش زنجیره ای پلیمراز واقعی در زمان واقعی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
MiRNAs are a novel group of non-coding small RNAs that negatively regulate gene expression. Many miRNAs have been identified and investigated extensively in plant species with sequenced genomes. However, few miRNAs have been identified in foxtail millet (Setaria italica), which is an ancient cereal crop of great importance for dry land agriculture. In this study, 271 foxtail millet miRNAs belonging to 44 families were identified using a bioinformatics approach. Twenty-three pairs of sense/antisense miRNAs belonging to 13 families, and 18 miRNA clusters containing members of 8 families were discovered in foxtail millet. We identified 432 potential targets for 38 miRNA families, most of which were predicted to be involved in plant development, signal transduction, metabolic pathways, disease resistance, and environmental stress responses. Gene ontology (GO) analysis revealed that 101, 56, and 23 target genes were involved in molecular functions, biological processes, and cellular components, respectively. We investigated the expression patterns of 43 selected miRNAs using qRT-PCR analysis. All of the miRNAs were expressed ubiquitously with many exhibiting different expression levels in different tissues. We validated five predicted targets of four miRNAs using the RNA ligase mediated rapid amplification of cDNA end (5â²-RLM-RACE) method.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 546, Issue 2, 10 August 2014, Pages 367-377
Journal: Gene - Volume 546, Issue 2, 10 August 2014, Pages 367-377
نویسندگان
Jun Han, Hao Xie, Qingpeng Sun, Jun Wang, Min Lu, Weixiang Wang, Erhu Guo, Jinbao Pan,