کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5905770 | 1159929 | 2014 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Partial cloning, tissue distribution and effects of epigallocatechin gallate on hepatic 3-hydroxy-3-methylglutaryl-CoA reductase mRNA transcripts in goldfish (Carassius auratus)
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کلمات کلیدی
SREBPSterol regulatory element-binding transcription factor 1CYP7A1SSDORFHMGCoARNADPHEGCGCarassius auratusppmRT-PCRABCA1MS-222SREBF1LXR3-hydroxy-3-methylglutaryl-CoA reductase - 3-هیدروکسی-3-methylglutaryl-CoA ردوکتازATP-binding cassette transporter A1 - ATP اتصال گیرنده کاست A1Real-time PCR - PCR به موقعExpression pattern - الگوی بیانepigallocatechin gallate - اپی گالوستاچین گالاتpart per million - بخشی در میلیونstandard error of the mean - خطای استاندارد میانگینsterol-sensing domain - دامنه سنجش استرولopen reading frame - قاب خواندن بازSEM - مدل معادلات ساختاری / میکروسکوپ الکترونی روبشیnicotinamide adenine dinucleotide phosphate - نیکوتین آمید adenine dinucleotide phosphatebody weight - وزن بدنsterol regulatory element binding protein - پروتئین اتصال دهنده عصاره استرولliver X receptor - کبد X گیرنده
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Epigallocatechin gallate (EGCG), the major active component of the green tea, has recently been found to inhibit 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCoAR) activity in vitro and to modulate lipogenesis in vivo. In this study we have evaluated the effects of short-term in vivo exposure to EGCG (6 μg gâ 1 BW or 9 μg gâ 1 BW) on hepatic HMGCoAR gene expression of goldfish (Carassius auratus). We initially characterized a partial sequence of goldfish HMGCoAR suggesting that the obtained fragment shares high similarity (> 92%) with other fish HMGCoAR sequences. Further, the HMGCoAR transcript was detected in all goldfish tissues (except muscle) but primarily in liver, brain and gonads; on the contrary, low expression levels were found in intestine, heart, gill, and kidney. Both EGCG doses significantly decreased hepatic HMGCoAR mRNA levels 180 min post-injection. HMGCoAR was also significantly down-regulated at 90 min after injection in fish treated with the highest dose of EGCG. Our results demonstrate that hepatic HMGCoAR gene expression is acutely responsive to short-term EGCG exposure in goldfish. This finding suggests a potential role of EGCG in transcriptional regulation of the rate-limiting enzyme in cholesterol synthesis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 545, Issue 2, 25 July 2014, Pages 220-225
Journal: Gene - Volume 545, Issue 2, 25 July 2014, Pages 220-225
نویسندگان
Paolo Cocci, Gilberto Mosconi, Francesco Alessandro Palermo,