کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5905790 | 1569846 | 2014 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effects of a novel SNP of IGF2R gene on growth traits and expression rate of IGF2R and IGF2 genes in gluteus medius muscle of Egyptian buffalo
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کلمات کلیدی
Man-6-PIGF2RIGF2SSCPHWERT-PCRSNPsADGχ2 - x2amino acid - آمینو اسیدeffective allele numbers - اعداد موثر آللEgyptian buffalo - بوفالو مصریHardy–Weinberg equilibrium - تعادل هاردی-وینبرگleast square means - حداقل مربع معنی استreverse transcription PCR - رونویسی معکوس PCRLSM - سازمان غیر دولتیinsulin-like growth factor 2 - فاکتور رشد مانند انسولین 2Month - ماهaverage daily gain - متوسط افزایش روزانهpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازsingle-strand conformation polymorphism - پلیمورفیسم یکپارچگیSingle nucleotide polymorphism - پلیمورفیسم تک نوکلئوتیدیSNP - چندریختی تک-نوکلئوتیدinsulin-like growth factor 2 receptor - گیرنده فاکتور رشد 2 مانند انسولین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Insulin-like growth factor 2 receptor (IGF2R) is responsible for degradation of the muscle development initiator, IGF2, and thus it can be used as a marker for selection strategies in the farm animals. The aim of this study was to search for polymorphisms in three coding loci of IGF2R, and to analyze their effect on the growth traits and on the expression levels of IGF2R and IGF2 genes in the gluteus medius muscle of Egyptian buffaloes. A novel A266C SNP was detected in the coding sequences of the third IGF2R locus (at nucleotide number 51 of exon 23) among Egyptian water buffaloes. This SNP was non-synonymous mutation and led to replacement of Y (tyrosine) amino acid (aa) by D (aspartic acid) aa. Three different single-strand conformation polymorphism patterns were observed in the third IGF2R locus: AA, AC, and CC with frequencies of 0.555, 0.195, and 0.250, respectively. Statistical analysis showed that the homozygous AA genotype significantly associated with the average daily gain than AC and CC genotypes from birth to 9Â mo of age. Expression analysis showed that the A266C SNP was correlated with IGF2, but not with IGF2R, mRNA levels in the gluteus medius muscle of Egyptian buffaloes. The highest IGF2 mRNA level was estimated in the muscle of animals with the AA homozygous genotype as compared to the AC heterozygotes and CC homozygotes. We conclude that A266C SNP at nucleotide number 51 of exon 23 of the IGF2R gene is associated with the ADG during the early stages of life (from birth to 9Â mo of age) and this effect is accompanied by, and may be caused by, increased expression levels of the IGF2 gene.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 540, Issue 2, 1 May 2014, Pages 133-139
Journal: Gene - Volume 540, Issue 2, 1 May 2014, Pages 133-139
نویسندگان
Mohammed Abu El-Magd, Haitham G. Abo-Al-Ela, Abeer El-Nahas, Ayman A. Saleh, Ali A. Mansour,