کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5905826 | 1159938 | 2014 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Unliganded estrogen receptor α stimulates bone sialoprotein gene expression
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
CREAP1ERαLUCGAPDHBSPFGFNTSFCSFRE - آزادیOsteoblasts - استئوبلاست هاEstrogen - استروژنBase pair(s) - جفت پایه (ها)minimum essential medium - حداقل حداقل مورد نیازTranscription - رونویسیfetal calf serum - سرم گوساله جنینbone sialoprotein - سیلوپروتئین استخوانcAMP response element - عنصر پاسخ cAMPfibroblast growth factor - فاکتور رشد فیبروبلاستluciferase - لوسیفرازMEM - مامانNucleotides - نوکلئوتیدactivator protein 1 - پروتئین فعال کننده 1glyceraldehydes-3-phosphate dehydrogenase - گلیسرالید هیدروژن 3-فسفات دهیدروژنازEstrogen receptor - گیرنده استروژنEstrogen receptor α - گیرنده استروژن α
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
To determine the molecular basis of the tissue-specific expression of BSP and its regulation by estrogen and the ER, we have analyzed the effects of β-estradiol and ERα on BSP gene transcription. ERα protein levels were increased after ERα overexpression in ROS17/2.8 cells. While BSP mRNA levels were increased by ERα overexpression, the endogenous and overexpressed BSP mRNA levels were not changed by β-estradiol (10â 8 M, 24 h). Luciferase activities of different sized BSP promoter constructs (pLUC3 ~ 6) were increased by ERα overexpression, whereas basal and induced luciferase activities by ERα overexpression were not influenced by β-estradiol. Effects of ERα overexpression were abrogated by 2 bp mutations in either the cAMP response element (CRE) or activator protein 1 (AP1)/glucocorticoid response element (GRE). Gel shift analyses showed that ERα overexpression increased binding to the CRE and AP1/GRE elements. Notably, the CRE-protein complexes were disrupted by ERα, CREB and phospho-CREB antibodies. The AP1/GRE-protein complexes were supershifted by the c-Fos antibody. These studies demonstrate that ERα stimulates BSP gene transcription in a ligand-independent manner by targeting the CRE and AP1/GRE elements in the rat BSP gene promoter.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 539, Issue 1, 10 April 2014, Pages 50-57
Journal: Gene - Volume 539, Issue 1, 10 April 2014, Pages 50-57
نویسندگان
Hideki Takai, Hiroyoshi Matsumura, Sari Matsui, Kyung Mi Kim, Masaru Mezawa, Yohei Nakayama, Yorimasa Ogata,