کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5906261 | 1159967 | 2013 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecular characterization and functional analysis of Toll-like receptor 3 gene in orange-spotted grouper (Epinephelus coioides)
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کلمات کلیدی
LRRJnkPAMPsEpinephelus coioidesJun N-terminal kinaseVNNTLR3PRRsNNVIHNVTLRs - TLR هاpathogen-associated molecular patterns - الگوهای مولکولی مرتبط با پاتوژنInnate immunity - ایمنی ذاتیGene expression - بیان ژنleucine-rich repeat - تکرار غنی از لوسینTIR - تیرViral nervous necrosis - نکروز عصبی ویروسیInfectious hematopoietic necrosis virus - ویروس نکروز خونسردی عفونیnervous necrosis virus - ویروس نکروز عصبیRNA virus - ویروسهای RNAPoly I:C - پلی I: CToll/interleukin-1 receptor - گیرنده Toll / interleukin-1pattern recognition receptors - گیرنده های تشخیص الگوToll-like receptors - گیرنده های پولی مانند
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Molecular characterization and functional analysis of Toll-like receptor 3 gene in orange-spotted grouper (Epinephelus coioides) Molecular characterization and functional analysis of Toll-like receptor 3 gene in orange-spotted grouper (Epinephelus coioides)](/preview/png/5906261.png)
چکیده انگلیسی
Toll-like receptor 3 (TLR3) plays an important role in activating innate immune responses during viral infection. In this report, TLR3 (EcTLR3) was characterized and analyzed for the first time in Epinephelus coioides. The full-length EcTLR3 cDNA is predicted to encode a 909 amino acid polypeptide that contains a signal peptide sequence, 18 leucine-rich repeat (LRR) motifs, a transmembrane region and a Toll/interleukin-1 receptor (TIR) domain. Quantitative real-time PCR revealed that the EcTLR3 mRNA was much more abundant in the liver than in other immune organs, and that the expression levels were very low in hemocyte and muscle. During development of the grouper, the levels of EcTLR3 transcripts increased with age, with very low expression levels at the early stages of development. EcTLR3 mRNA levels were examined in the liver at different times after treatment with polyriboinosinic polyribocytidylic acid (Poly I:C), and in nervous necrosis virus (NNV)-infected larval groupers. The results suggested that EcTLR3 plays an important role in a fish's defense against viral infection.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 527, Issue 1, 15 September 2013, Pages 174-182
Journal: Gene - Volume 527, Issue 1, 15 September 2013, Pages 174-182
نویسندگان
Kebing Lin, Hui Ge, Qi Lin, Jianshao Wu, Libin He, Qiongshan Fang, Chen Zhou, Minqiu Sun, Zhongchi Huang,