کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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5906886 | 1159991 | 2013 | 9 صفحه PDF | دانلود رایگان |
Picrorhiza kurrooa Royle ex Benth. is an economically important medicinal plant known to yield picrosides which have high medicinal value. Picroside I and picroside II are major picrosides associated with various bioactivities. The present work analyzed the expression of various genes of the picrosides biosynthesis pathway in different tissues of the plant in relation to the picrosides content. Eight full-length cDNA sequences namely, 1-deoxy-d-xylulose-5-phosphate synthase (2.317Â kb), 1-deoxy-d-xylulose-5-phosphate reductoisomerase (1.767Â kb), 4-diphosphocytidyl-2-C-methyl-d-erythritol kinase (1.674Â kb), 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (1.701Â kb), acetyl-CoA acetyltransferase (1.545Â kb), 3-hydroxy-3-methylglutaryl coenzyme A reductase (2.241Â kb), isopentenyl pyrophosphate isomerase (987Â bp) and geranyl diphosphate synthase (1.434Â kb), were cloned to full-length followed by expression analysis of ten genes vis-Ã -vis picrosides content analysis. There is maximum accumulation of picrosides in leaf tissue followed by the rhizome and root, and a similar pattern of expression was found in all the ten genes. The genes responded to the modulators of the picrosides biosynthesis. Picrosides accumulation was enhanced by application of hydrogen peroxide and abscisic acid, whereas methyl jasmonate and salicylic acid treatment decreased the content.
⺠Eight full-length cDNAs associated with picrosides biosynthesis were cloned. ⺠Younger leaves showed higher expression of genes as compared to mature leaves. ⺠Hydrogen peroxide and abscisic acid enhanced picrosides accumulation. ⺠Methyl jasmonate and salicylic acid decreased picrosides content. ⺠Expression of key genes was modulated in response to different modulators.
Journal: Gene - Volume 515, Issue 2, 25 February 2013, Pages 320-328