کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5907086 | 1159997 | 2013 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Short CommunicationInsulin-like factor binding protein-3 promotes the G1 cell cycle arrest in several cancer cell lines Short CommunicationInsulin-like factor binding protein-3 promotes the G1 cell cycle arrest in several cancer cell lines](/preview/png/5907086.png)
Insulin-like growth factor binding protein-3 (IGFBP-3) is a multi-functional protein known to induce apoptosis of various cancer cells in an insulin-like growth factor (IGF)-dependent and IGF-independent manner. In our previous study, we found that IGFBP-3 induced apoptosis through the activation of caspases in 786-O cells. In this study, we further examined that whether IGFBP-3 induced apoptosis through the induction of cell cycle arrest in 786-O, A549 and MCF-7 cells. Our results showed that overexpressed IGFBP-3 resulted in typical apoptotic ultrastructures in A549 cells under transmission electron microscope. The result of flow cytometry analysis indicated that IGFBP-3 arrested the cell cycle at G1-S phase in 786-O, A549 and MCF-7 cells. In A549 cells, quantitative real-time PCR and Western blot analysis showed a significant change in the expression of cell cycle-regulated proteins-a decrease in cyclin E1 expression, an increase in p21 expression. These results indicate a possible mechanism for G1 cell cycle arrest by IGFBP-3. Taken together, cyclin E1 and p21 may play important roles in the IGFBP-3-inducing G1 cell cycle arrest and apoptosis in several human cancer cells.
⺠IGFBP-3 resulted in typical apoptotic ultrastructures in A549 cells. ⺠IGFBP-3 could induce apoptosis in MCF-7 cells, especially the early apoptosis. ⺠IGFBP-3 arrested the cell cycle at G1-S phase in 786-O, A549 and MCF-7 cells. ⺠IGFBP-3 decreased the cyclinE1 expression and increased the p21 expression.
Journal: Gene - Volume 512, Issue 1, 1 January 2013, Pages 127-133