HER-2/neu gene amplification assessment in breast cancer patients in Isfahan province by real time PCR, differential PCR and immunohistochemistry

► The Her2/neu gene amplification has been assessed in 83 breast cancer patients. ► We have used real time PCR, differential PCR and immunohistochemistry. ► We have made a very precise comparison between these three different procedures. ► Very high concordances were seen between IHC and differential PCR or real time PCR. ► 55.5% concordance was seen between these three methods.