کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5907591 | 1569854 | 2011 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Sporophytic and gametophytic functions of the cell cycle-associated Mob1 gene in Arabidopsis thaliana L.
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کلمات کلیدی
Mps one binderTNEMTSBMMCDICSAVCDK1MOBMATSDAPI2-(N-morpholino)ethanesulfonic acid - 2- (N-مورفولینو) اتان سولفونیک اسید4',6-diamidino-2-phenylindole - 4 '، 6-دیامیدینو-2-فنیلینولBSA - BSAcDNA - cDNADMSO - DMSODNA complementary to RNA - DNA مکمل RNARNA interference - RNA تداخل کنندهRNAi - RNA سرکوبگر،RNA مداخلهگر، RNA خاموش کنندهbovine serum albumin - آلبومین سرم گاوFear - ترسPlant reproduction - تولید مثل گیاهیSeed set - دانه تنظیمDimethylsulfoxide - دیمتیل سولفواکسیدSalvador - سالوادورlarge tumor suppressor - سرکوب کننده تومور بزرگmitotic exit network - شبکه خروجی میتوزیLats - لاتMen - مردانMeS - مسUTR یا untranslated regions - منطقه ترجمه نشدهMeiosis - میوز Differential interference contrast microscopy - میکروسکوپ تضاد تداخل دیفرانسیلcyclin dependent kinase 1 - کیناز وابسته به سیکلین 1Gametogenesis - گامتوژنز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Mob1 genes are primarily involved in the cell cycle progression and mitosis exit in yeasts and animals. The function of a Mob1-like gene (At5g45550) from Arabidopsis thaliana was investigated using RNAi and immunological staining. AtMob1-like RNAi silenced lines showed a reduced radial expansion of the inflorescence stem and a reduced elongation zone of the primary root. Morphological features of plant organs were accompanied by a reduction in cell size. The fertility of AtMob1-like RNAi silenced lines was very low as seed production was strongly reduced. About 2% of the progeny of AtMob1-like RNAi silenced plants were tetraploid. The female and male sporogenesis was affected differentially. The ovules developed irregularly and one third of the megaspores and embryo sacs degenerated prematurely. Up to 20% of the ovules produced binucleated megaspores that failed to develop further, being their degeneration likely accompanied with a delayed programmed cell death. The anthers produced about 30% of aborted pollen grains, showing also a strong variation in their size. Together, the results show that Arabidopsis MOB1-like is required to regulate cell expansion and cell division, presumably by affecting the mitotic as well as the meiotic cell cycle.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 484, Issues 1â2, 15 September 2011, Pages 1-12
Journal: Gene - Volume 484, Issues 1â2, 15 September 2011, Pages 1-12
نویسندگان
Giulio Galla, Sara Zenoni, Gianpiero Marconi, Giada Marino, Alessandro Botton, Francesco Pinosa, Sandra Citterio, Benedetto Ruperti, Klaus Palme, Emidio Albertini, Mario Pezzotti, Martin Mau, Timothy F. Sharbel, Nico De Storme, Danny Geelen,