کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5907608 | 1569857 | 2011 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Unique sequences and predicted functions of myosins in Tetrahymena thermophila
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کلمات کلیدی
regulator of chromosome condensation 1Ras-related nuclear proteinFERMRCC1SH3F-actinTh1GEFqRT-PCRHMMGTPfilamentous actin - actin filamentousadenosine 5′-triphosphate - آدنوزین 5'-تری فسفاتATP - آدنوزین تری فسفات یا ATPactin - اکتین rapid amplification of cDNA ends - تقویت سریع cDNA به پایان می رسدGDP - تولید ناخالص ملیMolecular evolution - تکامل مولکولیRån - شتمGAP - شکافguanine nucleotide exchange factor - فاکتور تبادل نوکلئوتید گوانینHidden Markov model - مدل پنهان مارکوف Race - مسابقهUnconventional myosin - میوزین غیر متعارفSrc homology 3 - همبستگی Src 3quantitative real-time polymerase chain reaction - واکنش زنجیره ای پلیمراز کمی زمان واقعی استCiliate - پیگیریguanosine 5′-triphosphate - گوانوزین 5'-تری فسفاتguanosine 5′-diphosphate - گوانوزین 5'-دی فسفات
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Myosins are eukaryotic actin-dependent molecular motors that play important roles in many cellular events. The function of each myosin is determined by a variety of functional domains in its tail region. In some major model organisms, the functions and properties of myosins have been investigated based on their amino acid sequences. However, in protists, myosins have been little studied beyond the level of genome sequences. We therefore investigated the mRNA expression levels and amino acid sequences of 13 myosin genes in the ciliate Tetrahymena thermophila. This study is an overview of myosins in T. thermophila, which has no typical myosins, such as class I, II, or V myosins. We showed that all 13 myosins were expressed in vegetative cells. Furthermore, these myosins could be divided into 3 subclasses based on four functional domains in their tail regions. Subclass 1 comprised of 8 myosins has both MyTH4 and FERM domains, and has a potential to function in vesicle transport or anchoring between membrane and actin filaments. Subclass 2 comprised of 4 myosins has RCC1 (regulator of chromosome condensation 1) domains, which are found only in some protists, and may have unconventional features. Subclass 3 is comprised of one myosin, which has a long coiled-coil domain like class II myosin. In addition, phylogenetic analysis on the basis of motor domains showed that T. thermophila myosins are separated into two clusters: one consists of subclasses 1 and 2, and the other consists of subclass 3.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 480, Issues 1â2, 1 July 2011, Pages 10-20
Journal: Gene - Volume 480, Issues 1â2, 1 July 2011, Pages 10-20
نویسندگان
Maki Sugita, Yoshinori Iwataki, Kentaro Nakano, Osamu Numata,