کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5907634 | 1569919 | 2008 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Sequencing, bioinformatic characterization and expression pattern of a putative amino acid transporter from the parasitic cestode Echinococcus granulosus
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کلمات کلیدی
ASCTBCIPNBTSSTPBSTSDFSSCORFTween-20TrxPBSBSA - BSAcDNA - cDNADNA complementary to RNA - DNA مکمل RNAbovine serum albumin - آلبومین سرم گاوHelminth parasites - انگل HelminthCystic echinococcosis - اکینوکوکوز کیستیکnitro blue tetrazolium - تترازولیم نیترو آبیReverse transcriptase - ترانس کریپتاز معکوس یا وارونویسELISA - تست الیزاEnzyme-linked immunosorbent assay - تست الیزاrapid amplification of cDNA ends - تقویت سریع cDNA به پایان می رسدSignal sequence trap - تله دنباله سیگنالsodium saline citrate - سدیم سدیم سیتراتopen reading frame - قاب خواندن بازPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریRace - مسابقهUTR یا untranslated regions - منطقه ترجمه نشدهuntranslated region - منطقه غیر ترجمهpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمراز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Sequencing, bioinformatic characterization and expression pattern of a putative amino acid transporter from the parasitic cestode Echinococcus granulosus Sequencing, bioinformatic characterization and expression pattern of a putative amino acid transporter from the parasitic cestode Echinococcus granulosus](/preview/png/5907634.png)
چکیده انگلیسی
We have sequenced and partially characterized an Echinococcus granulosus cDNA, termed egat1, from a protoscolex signal sequence trap (SST) cDNA library. The isolated 1627Â bp long cDNA contains an ORF of 489 amino acids and shows an amino acid identity of 30% with neutral and excitatory amino acid transporters members of the Dicarboxylate/Amino Acid Na+ and/or H+ Cation Symporter family (DAACS) (TC 2.A.23). Additional bioinformatics analysis of EgAT1, confirmed the results obtained by similarity searches and showed the presence of 9 to 10 transmembrane domains, consensus sequences for N-glycosylation between the third and fourth transmembrane domain, a highly similar hydropathy profile with ASCT1 (a known member of DAACS family), high score with SDF (Sodium Dicarboxilate Family) and similar motifs with EDTRANSPORT, a fingerprint of excitatory amino acid transporters. The localization of the putative amino acid transporter was analyzed by in situ hybridization and immunofluorescence in protoscoleces and associated germinal layer. The in situ hybridization labelling indicates the distribution of egat1 mRNA throughout the tegument. EgAT1 protein, which showed in Western blots a molecular mass of ~Â 60Â kD, is localized in the subtegumental region of the metacestode, particularly around suckers and rostellum of protoscoleces and layers from brood capsules. The sequence and expression analyses of EgAT1 pave the way for functional analysis of amino acids transporters of E. granulosus and its evaluation as new drug targets against cystic echinococcosis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 411, Issues 1â2, 31 March 2008, Pages 1-9
Journal: Gene - Volume 411, Issues 1â2, 31 March 2008, Pages 1-9
نویسندگان
Federico Camicia, Rodolfo Paredes, Cora Chalar, Norbel Galanti, Laura Kamenetzky, Ariana Gutierrez, Mara C. Rosenzvit,