کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5908155 1570159 2016 28 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Investigating intra-host and intra-herd sequence diversity of foot-and-mouth disease virus
ترجمه فارسی عنوان
بررسی توالی درون میزبان و درون گله از بیماری ویروس آنفلوآنزای مرغی
کلمات کلیدی
مرض پا و دهان، توالی نسل بعدی، تجزیه و تحلیل اختیاری، فیلوژنتیک، تنوع ویروسی،
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک بوم شناسی، تکامل، رفتار و سامانه شناسی
چکیده انگلیسی
Due to the poor-fidelity of the enzymes involved in RNA genome replication, foot-and-mouth disease (FMD) virus samples comprise of unique polymorphic populations. In this study, deep sequencing was utilised to characterise the diversity of FMD virus (FMDV) populations in 6 infected cattle present on a single farm during the series of outbreaks in the UK in 2007. A novel RT-PCR method was developed to amplify a 7.6 kb nucleotide fragment encompassing the polyprotein coding region of the FMDV genome. Illumina sequencing of each sample identified the fine polymorphic structures at each nucleotide position, from consensus level changes to variants present at a 0.24% frequency. These data were used to investigate population dynamics of FMDV at both herd and host levels, evaluate the impact of host on the viral swarm structure and to identify transmission links with viruses recovered from other farms in the same series of outbreaks. In 7 samples, from 6 different animals, a total of 5 consensus level variants were identified, in addition to 104 sub-consensus variants of which 22 were shared between 2 or more animals. Further analysis revealed differences in swarm structures from samples derived from the same animal suggesting the presence of distinct viral populations evolving independently at different lesion sites within the same infected animal.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Infection, Genetics and Evolution - Volume 44, October 2016, Pages 286-292
نویسندگان
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