کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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59085 | 1419452 | 2013 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Biomimetic preparation of organic-inorganic composite microcapsules for glucose oxidase immobilization Biomimetic preparation of organic-inorganic composite microcapsules for glucose oxidase immobilization](/preview/png/59085.png)
Organic-inorganic composite microcapsules made of sodium poly(styrenesulfonate) (PSS), poly(dimethyl diallyl ammonium chloride) (PDADMAC), and biomimetic silica were successfully fabricated via a combination of layer-by-layer self-assembly and biomimetic mineralization. The morphologies and chemical compositions of the microcapsules (denoted by (PSS/PDADMAC)2-SiO2) were characterized using scanning electron microscopy, Fourier-transform infrared spectroscopy, and thermogravimetric analysis. The composite microcapsules were used as carrier for the immobilization of glucose oxidase (GOD). GOD encapsulated in (PSS/PDADMAC)2-SiO2 (denoted by GOD@(PSS/PDADMAC)2-SiO2) displayed improved thermal, pH, and operational stabilities. The activity recovery of the immobilized GOD reached 72.85% under the optimized conditions. GOD@(PSS/PDADMAC)2-SiO2 showed a 2.21-fold increase in the Michaelis constant (Km) compared with free GOD. The composite microcapsules have the potential for a range of applications in chemical/biological catalysis and drug/gene delivery systems, and as biosensors.
Graphical AbstractGlucose oxidase (GOD) was immobilized in organic-inorganic composite microcapsules using a combination of layer-by-layer assembly and biomimetic mineralization. The encapsulated GOD exhibited distinct advantages in terms of thermal, pH, and operational stabilities.Figure optionsDownload as PowerPoint slide
Journal: Chinese Journal of Catalysis - Volume 34, Issue 8, August 2013, Pages 1627–1633