کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5908724 1570164 2016 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Complete genome sequencing of a recombinant strain between human astrovirus antigen types 2 and 8 isolated from South Korea
ترجمه فارسی عنوان
توالی ژنوم کامل یک سوند نوترکیب بین انواع آنتی ژن های آسترو ویروس 2 و 8 جدا شده از کره جنوبی
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک بوم شناسی، تکامل، رفتار و سامانه شناسی
چکیده انگلیسی
Human astroviruses (HAstVs) occur worldwide and are known to the causative agents of diarrhea in infants and elderly patients with immune dysfunction. This study aimed to identify recombinant HAstV strains and characterize rare genotypes. The full-length genome of a recombinant HAstV strain isolated from the stool sample of a patient with acute gastroenteritis from South Korea was amplified using three pairs of previously designed primers and seven newly designed primers. The recombinant HAstV was 6757-bp long and contained three sequential open reading frames (ORFs), designated as ORF1a (2781 bp), ORF1b (1548 bp), and ORF2 (2349 bp). Our findings suggested that a recombination event had occurred between ORF1b and ORF2 of the isolated strain, with a recombination breakpoint at 4081 bp. To our knowledge, this is the first study to reveal the complete nucleotide sequence of a recombinant HAstV strain from South Korea. Our study findings might be useful for identifying other recombinant HAstV strains and for developing vaccines against this pathogenic virus.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Infection, Genetics and Evolution - Volume 39, April 2016, Pages 127-131
نویسندگان
, , , , , ,