کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5910802 1161356 2012 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Implication of the RDRioMycobacterium tuberculosis sublineage in multidrug resistant tuberculosis in Portugal
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک بوم شناسی، تکامل، رفتار و سامانه شناسی
پیش نمایش صفحه اول مقاله
Implication of the RDRioMycobacterium tuberculosis sublineage in multidrug resistant tuberculosis in Portugal
چکیده انگلیسی

Multidrug and extensively drug resistant Mycobacterium tuberculosis are a threat to tuberculosis control programs. Genotyping methods, such as spoligotyping and MIRU-VNTR typing (Mycobacterial Interspersed Repetitive Units), are useful in monitoring potentially epidemic strains and estimating strain phylogenetic lineages and/or genotypic families. M. tuberculosis Latin American Mediterranean (LAM) family is a major worldwide contributor to tuberculosis (TB). LAM specific molecular markers, Ag85C103 single nucleotide polymorphism (SNP) and RDRio long-sequence polymorphism (LSP), were used to characterize spoligotype signatures from 859 patient isolates from Portugal. LAM strains were found responsible for 57.7% of all tuberculosis cases. Strains with the RDRio deletion (referred to as RDRio) were estimated to represent 1/3 of all the strains and over 60% of the multidrug resistant (MDR) strains. The major spoligotype signature SIT20 belonging to the LAM1 RDRio sublineage, represented close to 1/5th of all the strains, over 20% of which were MDR. Analysis of published datasets according to stipulated 12 loci MIRU-VNTR RDRio signatures revealed that 96.3% (129/134) of MDR and extensively drug resistant (XDR) clusters were RDRio. This is the first report associating the LAM RDRio sublineage with MDR. These results are an important contribution to the monitoring of these strains with heightened transmission for future endeavors to arrest MDR-TB and XDR-TB.

► LAM strains caused 57.7% of the tuberculosis cases in this Portuguese study. ► The RDRio sublineage represented 1/3 of the strains and over 60% of the MDR strains. ► Spoligotype signature SIT20 (RDRio), representing 1/5th of the strains, was 20% MDR. ► Analysis of published datasets revealed that 96.3% MDR and XDR clusters were RDRio. ► This is the first report associating the LAM RDRio sublineage with MDR-TB.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Infection, Genetics and Evolution - Volume 12, Issue 7, October 2012, Pages 1362-1367
نویسندگان
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