A nuclear single-nucleotide polymorphism (SNP) potentially useful for the separation of Rhodnius prolixus from members of the Rhodnius robustus cryptic species complex (Hemiptera: Reduviidae)

- Accurate species identification is key for effective vector control of Chagas disease.
- Rhodnius prolixus is an efficient domestic vector and R. robustus s.l. is sylvatic.
- These species are cryptic, can co-occur and occasionally hybridize in nature.
- We describe a SNP in a scnDNA that separates R. prolixus from R. robustus s.l.
- This marker will allow for the detection of crossbreeding and mtDNA introgression.