کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5911086 | 1570185 | 2013 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Molecular epidemiology of American/Asian genotype DENV-2 in Peru Molecular epidemiology of American/Asian genotype DENV-2 in Peru](/preview/png/5911086.png)
- We performed phylogenetic analyses based on the DENV-2 E gene sequence.
- Sequences from Peru and neighboring countries from the past decade were obtained.
- Four clades of DENV-2 American/Asian genotype were identified in Peru.
- Two routes of entry into Peru were identified for the different clades.
- Lineage II-clade F was associated with a recent DHF epidemic in northeastern Peru.
During the past decade, countries in South America have reported dengue hemorrhagic fever (DHF) associated with American/Asian genotype of dengue virus serotype 2 (DENV-2). DENV-2 strains have been associated with large outbreaks of dengue fever and DHF in numerous regions of Peru since the mid-1990s, but studies to address the origins, distribution, and genetic diversity of DENV-2 strains have been limited. To address this knowledge gap, we sequenced the envelope gene region of DENV-2 isolates from Peru, Ecuador, Paraguay, and Bolivia. Sequences were aligned and compared to a global sample of DENV-2 viruses. Phylogenetic analysis confirmed the circulation of two DENV-2 genotypes in Peru: American (prior to 2001) and American/Asian (2000 to present). American/Asian genotype variants can be classified into two lineages, and these were introduced into Peru from the north (Ecuador, Colombia, and/or Venezuela) and the east (Brazil and Bolivia). American/Asian lineage II replaced lineage I after 2009. We estimate the time to the most recent common ancestor for American/Asian DENV-2 genotype in the Americas was in 1980, and 1984 and 1989 for lineages I and II, respectively. In light of evidence for increased virulence of lineage II of American/Asian DENV-2, our results support the need for continuous monitoring for the emergence of new DENV genotypes that may be associated with severe disease.
Journal: Infection, Genetics and Evolution - Volume 18, August 2013, Pages 220-228