Determination of drug susceptibility patterns and genotypes of Mycobacterium tuberculosis isolates from Kanpur district, North India

► To characterize Mycobacterium tuberculosis genotypes in Kanpur district, India by spoligotyping and IS6110-RFLP. ► Predominant spoligotypes belonged to Central Asian Delhi family (33.78%) and Beijing family (10.8%). ► All isolates clustered by spoligotyping were identified unique by RFLP. ►Eleven (14.86%) were multi drug-resistant and out of them, 6 were of ST1/Beijing family. ► MDR-TB was significantly higher in Beijing strain (p < 0.0001).