The peptidoglycan hydrolase TcpG is required for efficient conjugative transfer of pCW3 in Clostridium perfringens

► pCW3 is the paradigm conjugative plasmid in Clostridium perfringens. ► Mutations were constructed in the pCW3-encoded tcpG and tcpI genes. ► tcpG was shown to be essential for high frequency conjugative transfer of pCW3. ► TcpG was shown to have peptidoglycan hydrolase-like activity.