کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5914059 | 1162719 | 2014 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Your personalized protein structure: Andrei N. Lupas fused to GCN4 adaptors Your personalized protein structure: Andrei N. Lupas fused to GCN4 adaptors](/preview/png/5914059.png)
This work presents a protein structure that has been designed purely for aesthetic reasons, symbolizing decades of coiled-coil research and praising its most fundamental model system, the GCN4 leucine zipper. The GCN4 leucine zipper is a highly stable coiled coil which can be tuned to adopt different oligomeric states via mutation of its core residues. For these reasons it is used in structural studies as a stabilizing fusion adaptor. On the occasion of the 50th birthday of Andrei N. Lupas, we used it to create the first personalized protein structure: we fused the sequence ANDREI-N-LVPAS in heptad register to trimeric GCN4 adaptors and determined its structure by X-ray crystallography. The structure demonstrates the robustness and versatility of GCN4 as a fusion adaptor. We learn how proline can be accommodated in trimeric coiled coils, and put the structure into the context of the other GCN4-fusion structures known to date.
Journal: Journal of Structural Biology - Volume 186, Issue 3, June 2014, Pages 380-385