کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5914263 1162726 2014 14 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A marker-free automatic alignment method based on scale-invariant features
ترجمه فارسی عنوان
روش تطبیق خودکار بدون مارکر براساس ویژگیهای مقیاس ناپایدار
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی مولکولی
چکیده انگلیسی
In electron tomography, alignment accuracy is critical for high-resolution reconstruction. However, the automatic alignment of a tilt series without fiducial markers remains a challenge. Here, we propose a new alignment method based on Scale-Invariant Feature Transform (SIFT) for marker-free alignment. The method covers the detection and localization of interest points (features), feature matching, feature tracking and optimization of projection parameters. The proposed method implements a highly reliable matching strategy and tracking model to detect a huge number of feature tracks. Furthermore, an incremental bundle adjustment method is devised to tolerate noise data and ensure the accurate estimation of projection parameters. Our method was evaluated with a number of experimental data, and the results exhibit an improved alignment accuracy comparable with current fiducial marker alignment and subsequent higher resolution of tomography.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Structural Biology - Volume 186, Issue 1, April 2014, Pages 167-180
نویسندگان
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