کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5914320 1162731 2014 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Heparin induced dimerization of APP is primarily mediated by E1 and regulated by its acidic domain
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
Heparin induced dimerization of APP is primarily mediated by E1 and regulated by its acidic domain
چکیده انگلیسی
The amyloid precursor protein (APP) and its cellular processing are believed to be centrally involved in the etiology of Alzheimer's disease (AD). In addition, many physiological functions have been described for APP, including a role in cell-cell- and cell-ECM-adhesion as well as in axonal outgrowth. We show here the molecular determinants of the oligomerization/dimerization of APP, which is central for its cellular (mis)function. Using size exclusion chromatography (SEC), dynamic light scattering and SEC-coupled static light scattering we demonstrate that the dimerization of APP is energetically induced by a heparin mediated dimerization of the E1 domain, which results in a dimeric interaction of E2. We also show that the acidic domain (AcD) interferes with the dimerization of E1 and propose a model where both, cis- and trans-dimerization occur dependent on cellular localization and function.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Structural Biology - Volume 187, Issue 1, July 2014, Pages 30-37
نویسندگان
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