کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5914433 | 1162740 | 2012 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cryo-electron tomography and 3-D analysis of the intact flagellum in Trypanosoma brucei
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Cryo-electron tomography and 3-D analysis of the intact flagellum in Trypanosoma brucei Cryo-electron tomography and 3-D analysis of the intact flagellum in Trypanosoma brucei](/preview/png/5914433.png)
چکیده انگلیسی
Trypanosoma brucei is a uni-cellular protist that causes African sleeping sickness. These parasites have a flagellum that is attached to the cell body and is indispensible for its motility. The flagellum consists of a canonical 9Â +Â 2 axoneme and a paraflagellar rod (PFR), an intricate tripartite, fibrous structure that is connected to the axoneme. In this paper we describe results from cryo-electron tomography of unperturbed flagella. This method revealed novel structures that are likely involved in attaching the flagellum to the cell. We also show the first cryo-electron tomographic images of a basal body in situ, revealing electron dense structures inside its triplet microtubules. Sub-tomogram averaging of the PFR revealed that its distal region is organized as an orthorhombic crystal.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Structural Biology - Volume 178, Issue 2, May 2012, Pages 189-198
Journal: Journal of Structural Biology - Volume 178, Issue 2, May 2012, Pages 189-198
نویسندگان
Johanna L. Höög, Cédric Bouchet-Marquis, J. Richard McIntosh, Andreas Hoenger, Keith Gull,