کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5914480 1162741 2012 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Super-resolution fluorescence imaging of chromosomal DNA
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
Super-resolution fluorescence imaging of chromosomal DNA
چکیده انگلیسی
Super-resolution microscopy is a powerful tool for understanding cellular function. However one of the most important biomolecules - DNA - remains somewhat inaccessible because it cannot be effectively and appropriately labeled. Here, we demonstrate that robust and detailed super-resolution images of DNA can be produced by combining 5-ethynyl-2′-deoxyuridine (EdU) labeling using the 'click chemistry' approach and direct stochastic optical reconstruction microscopy (dSTORM). This method can resolve fine chromatin structure, and - when used in conjunction with pulse labeling - can reveal the paths taken by individual fibers through the nucleus. This technique should provide a useful tool for the study of nuclear structure and function.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Structural Biology - Volume 177, Issue 2, February 2012, Pages 344-348
نویسندگان
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