کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5914788 | 1162754 | 2011 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A differential structure approach to membrane segmentation in electron tomography
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی مولکولی
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چکیده انگلیسی
Electron tomography allows three-dimensional visualization of cellular landscapes in molecular detail. Segmentation is a paramount stage for the interpretation of the reconstructed tomograms. Although several computational approaches have been proposed, none has prevailed as a generic method and thus segmentation through manual annotation is still a common choice. In this work we introduce a segmentation method targeted at membranes, which define the natural limits of compartments within biological specimens. Our method is based on local differential structure and on a Gaussian-like membrane model. First, it isolates information through scale-space and finds potential membrane-like points at a local scale. Then, the structural information is integrated at a global scale to yield the definite segmentation. We show and validate the performance of the algorithm on a number of tomograms under different experimental conditions.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Structural Biology - Volume 175, Issue 3, September 2011, Pages 372-383
Journal: Journal of Structural Biology - Volume 175, Issue 3, September 2011, Pages 372-383
نویسندگان
Antonio Martinez-Sanchez, Inmaculada Garcia, Jose-Jesus Fernandez,