کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5915600 | 1163313 | 2012 | 4 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Short communicationEvidence for filamentous actin in ookinetes of a malarial parasite Short communicationEvidence for filamentous actin in ookinetes of a malarial parasite](/preview/png/5915600.png)
Extracellular stages of apicomplexan parasites utilize their own actin myosin motor machinery for gliding locomotion, penetration of cell barriers, and host cell invasion. Thus far, filamentous actin could not be visualized by standard microscopic techniques in vivo. Here, we describe the generation of a novel peptide antibody against the divergent amino-terminal portion of the major Plasmodium isoform, actin I. We show that our antiserum, termed Ab-actinI-I, is conformation-specific. In motile ookinetes it recognizes actin in rod-like structures, which are sensitive to inhibitors interfering with actin polymerization. The average size of the rods is 600Â nm, which is considerably longer than what has been detected in in vitro studies of actin filaments.
192Highlights⺠Recognition of filament-like actin rods in malarial ookinetes. ⺠Length of the actin-staining rod is â¼600 nm. ⺠Actin inhibitors support conformational specificity of anti-actin I antiserum.
Journal: Molecular and Biochemical Parasitology - Volume 181, Issue 2, February 2012, Pages 186-189