کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5915825 1163333 2010 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Short technical reportEchinococcus multilocularis primary cells: Improved isolation, small-scale cultivation and RNA interference
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
Short technical reportEchinococcus multilocularis primary cells: Improved isolation, small-scale cultivation and RNA interference
چکیده انگلیسی

In this study we demonstrate RNA interference mediated knock-down of target gene expression in Echinococcus multilocularis primary cells on both the transcriptional and translational level. In addition, we report on an improved method for generating E. multilocularis primary cell mini-aggregates from in vitro cultivated metacestode vesicles, and on the cultivation of small numbers of small interfering RNA-transfected cells in vitro over an extended period of time. This allows assessments on the effects of RNA interference performed on Echinococcus primary cells with regard to growth, proliferation, differentiation of the parasite and the formation of novel metacestode vesicles in vitro.

108Research highlights▶ Echinococcus multilocularis primary cells form highly viable mini-aggregates in vitro. ▶ Echinococcus mini-aggregates can be cultured in vitro for extended periods of time. ▶ RNAi was established to modulate gene expression in Echinococcus mini-aggregates.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Biochemical Parasitology - Volume 174, Issue 1, November 2010, Pages 83-87
نویسندگان
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