کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5915988 | 1163353 | 2008 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Expression and substrate specificity of a recombinant cysteine proteinase B of Leishmania braziliensis
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی مولکولی
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چکیده انگلیسی
The cysteine proteinase B of Leishmania parasites is an important virulence factor. In this study we have expressed, isolated and characterized for the first time a recombinant CPB from Leishmania braziliensis, the causative agent of mucocutaneous leishmaniosis. The mature region of the recombinant CPB shares a high percentage identity with its Leishmania mexicana CPB2.8 (rCPB2.8ÎCTE) counterpart (76.36%) and has identical amino acid residues at the S1, catalytic triad and Sâ²1 subsites. Nevertheless, when the kinetics of substrate hydrolysis was measured using a combinatorial library of internally quenched fluorescent peptides based upon the lead sequence Abz-KLRSSKQ-EDDnp, significant differences were obtained. These results suggest that the differences in substrate utilization observed between the L. mexicana and L. braziliensis CPs must be related to amino acid modifications outside the core of the active site cleft. Moreover, a potent inhibitor with Pro at P1 and high affinity for L. braziliensis recombinant CPB showed less affinity to L. mexicana CPB 2.8, which preferred Phe, Leu, and Asn at the same position.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Biochemical Parasitology - Volume 161, Issue 2, October 2008, Pages 91-100
Journal: Molecular and Biochemical Parasitology - Volume 161, Issue 2, October 2008, Pages 91-100
نویسندگان
Maria F. Lanfranco, Raúl Loayza-Muro, Daniel Clark, Regina Núñez, Amparo I. Zavaleta, Maribel Jimenez, Morten Meldal, Graham H. Coombs, Jeremy C. Mottram, Mario Izidoro, Maria A. Juliano, Luiz Juliano, Jorge Arévalo,