کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5916015 1570659 2008 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Sex- and stage-specific reporter gene expression in Plasmodium falciparum
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
Sex- and stage-specific reporter gene expression in Plasmodium falciparum
چکیده انگلیسی
For malaria transmission, Plasmodium parasites must successfully complete gametocytogenesis in the vertebrate host. Differentiation into mature male or female Plasmodium falciparum gametocytes takes 9-12 days as the parasites pass through five distinct morphologic stages (I-V). To evaluate the signals controlling the initiation of stage- and/or sex-specific expression, reporter constructs containing the 5′-flanking regions (FR) of seven genes with distinct expression patterns through gametogenesis were developed. The regulatory information present in the 5′-FR of each selected gene was found to be sufficient to drive appropriate sex- and stage-specific reporter gene expression. The transformed parasite lines also provide in vivo markers to identify gametocytes at specific stages, including a subpopulation of schizonts that express early gametocyte markers.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Biochemical Parasitology - Volume 160, Issue 2, August 2008, Pages 148-151
نویسندگان
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