کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5916356 1570718 2016 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Esterase D enhances type I interferon signal transduction to suppress foot-and-mouth disease virus replication
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
Esterase D enhances type I interferon signal transduction to suppress foot-and-mouth disease virus replication
چکیده انگلیسی


- FMDV infection induces the expression of esterase D (ESD) in PK-15 cells.
- ESD restricts FMDV replication in PK-15 cells.
- ESD enhances IFN-β signal transduction through promoting phosphorylation of IRF3.
- ESD suppresses FMDV replication through upregulation of various interferon-stimulated genes (ISGs).

The enzymatic activities of esterase D (ESD) are involved in many human diseases. However, no antiviral property of ESD has been described to date. Foot-and-mouth disease virus (FMDV) is the etiological agent of foot-and-mouth disease. In this study, we showed that FMDV infection triggered ESD expression. Overexpression of ESD significantly suppressed FMDV replication and knockdown of ESD expression enhanced virus replication, showing an essential antiviral role of ESD. Furthermore, we found that Sendai-virus-induced interferon (IFN) signaling was enhanced by upregulation of ESD, and ESD promoted activation of the IFN-β promoter simulated by IFN regulatory factor (IRF)3 or its upstream molecules (retinoic acid-inducible gene-I, melanoma differentiation-associated protein 5, virus-induced signaling adaptor and TANK binding kinase 1). Detailed analysis revealed that ESD protein enhanced IRF3 phosphorylation during FMDV infection. Overexpression of ESD also promoted the expression of various antiviral interferon-stimulated genes (ISGs) and knockdown of ESD impaired the expression of these antiviral genes during FMDV infection. Our findings demonstrate a new mechanism evolved by ESD to enhance type I IFN signal transduction and suppress viral replication during FMDV infection.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular Immunology - Volume 75, July 2016, Pages 112-121
نویسندگان
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