کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5916623 | 1163753 | 2015 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
ZC3H12D attenuated inflammation responses by reducing mRNA stability of proinflammatory genes
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کلمات کلیدی
LPSqPCRTLRAcute lung injury - آسیب ریه حادinflammation - التهاب( توروم) Ali - اماBALF - بافتToll-like receptor - تیالآرmRNA stability - ثبات mRNALipopolysaccharides - لیپوپلی ساکارید هاBronchoalveolar lavage fluid - مایع لارو برونکلوفلورUTR یا untranslated regions - منطقه ترجمه نشدهuntranslated region - منطقه غیر ترجمهquantitative real-time polymerase chain reaction - واکنش زنجیره ای پلیمراز کمی زمان واقعی است
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Infection in airspaces and lung parenchyma may cause acute lung injury and multiple organ dysfunction syndrome due to acute inflammatory response, leading to organ failure and high mortality. ZC3H12D has been shown to modulate Toll-like receptor signaling. This study aimed to investigate the change of ZC3H12D during acute lung injury and its role in inflammation processes. Mice were challenged with lipopolysaccharides (LPS) intratracheally. The expression levels of Zc3h12d, NF-κB, and cytokines were analyzed by quantitative real-time PCR (qPCR), ELISA, and Western blot. The mRNA stability was assessed by qPCR after cells were treated with actinomycin D for specified times. The 3â² untranslated region (3â²-UTR) of c-fos was cloned immediately downstream of the luciferase coding sequence driven by CMV promoter and luciferase activity was measured with a Luciferase Assay kit. Upon LPS treatment, ZC3H12D levels were reduced in mouse immune cells, whereas levels of NF-κB, IL-6, and TNF-α were significantly increased. Knockdown Zc3h12d in THP1 cells resulted in the upregulation of NF-κB while overexpression of Zc3h12d inhibited NF-κB expression. Ectopic Zc3h12d significantly reduced the mRNA stability of c-fos, NF-κB, TNF-α, IL-1β, and IL-6. Attachment of the c-fos 3â²-UTR made luciferase expression levels sensitive to levels of ZC3H12D. The data indicated that ZC3H12D could suppress both the initial inflammation storm and chronic inflammation by targeting the mRNA of cytokines as well as NF-κB and c-fos.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular Immunology - Volume 67, Issue 2, Part B, October 2015, Pages 206-212
Journal: Molecular Immunology - Volume 67, Issue 2, Part B, October 2015, Pages 206-212
نویسندگان
Hong Zhang, Wen-chen Wang, Jia-kuan Chen, Lin Zhou, Ming Wang, Zhen-dong Wang, Bo Yang, Yan-ming Xia, Shi Lei, En-qing Fu, Tao Jiang,