کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5916770 1163756 2014 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Differential IgE binding to isoallergens from Asian seabass (Lates calcarifer) in children and adults
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
Differential IgE binding to isoallergens from Asian seabass (Lates calcarifer) in children and adults
چکیده انگلیسی
The aim of this study was to identify and characterize allergenic proteins from barramundi in both fish allergic adult and pediatric patients. Serum from 17 fish allergic adults and children from Australia were characterized by immunoblotting and enzyme linked immunosorbent assays (ELISA) against raw and heated barramundi. Molecular analysis of identified allergens included genetic sequencing and generation of recombinant isoallergens. Two novel parvalbumin isoforms of the β-type were identified as the only allergens in barramundi and subsequently designated as Lat c 1.0101 and Lat c 1.0201 by the International Union of Immunological Societies. These two isoallergens do not differ in their ability to bind IgE antibodies, but are differentially expressed in barramundi tissue. This study characterized two novel heat stable parvalbumin allergens from barramundi, with differential IgE binding capacity between adults and pediatric patients.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular Immunology - Volume 62, Issue 1, November 2014, Pages 77-85
نویسندگان
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