کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5917030 | 1163768 | 2014 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cyclic AMP synergizes with butyrate in promoting β-defensin 9 expression in chickens
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
HDPHDACiAP-1VREforskolindb-cAMPICERVDRFskdibutyryl-cAMPDDA2′,5′-dideoxyadenosineColeus forskohliiGAPDHCRECREBcAMP - cAMPMAPK - MAPKCyclic adenosine monophosphate - آدنوزین مونوفسفات CyclicButyrate - بوتیراتChickens - جوجه هاInducible cAMP early repressor - سرکوب کننده اولیه cAMP قابل انعطاف استpertussis toxin - سموم سورافنیcholera toxin - سوزش گوارشیcAMP signaling - سیگنال cAMPcAMP response element - عنصر پاسخ cAMPDisease resistance - مقاومت در برابر بیماریhistone deacetylase inhibitor - مهار کننده هیستون داسیدلازCRE-binding protein - پروتئین CRE-bindingactivator protein-1 - پروتئین فعال کننده-1mitogen-activated protein kinase - پروتئین کیناز فعال با mitogenhost defense peptide - پپتید دفاع میزبانglyceraldehyde-3-phosphate dehydrogenase - گلیسرالیدید-3-فسفات دهیدروژنازVitamin D receptor - گیرنده ویتامین D
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Host defense peptides (HDP) have both microbicidal and immunomodulatory properties. Specific induction of endogenous HDP synthesis has emerged as a novel approach to antimicrobial therapy. Cyclic adenosine monophosphate (cAMP) and butyrate have been implicated in HDP induction in humans. However, the role of cAMP signaling and the possible interactions between cAMP and butyrate in regulating HDP expression in other species remain unknown. Here we report that activation of cAMP signaling induces HDP gene expression in chickens as exemplified by β-defensin 9 (AvBD9). We further showed that, albeit being weak inducers, cAMP agonists synergize strongly with butyrate or butyrate analogs in AvBD9 induction in macrophages and primary jejunal explants. Additionally, oral supplementation of forskolin, an adenylyl cyclase agonist in the form of a Coleus forskohlii extract, was found to induce AvBD9 expression in the crop of chickens. Furthermore, feeding with both forskolin and butyrate showed an obvious synergy in triggering AvBD9 expression in the crop and jejunum of chickens. Surprisingly, inhibition of the MEK-ERK mitogen-activated protein kinase (MAPK) pathway augmented the butyrate-FSK synergy, whereas blocking JNK or p38 MAPK pathway significantly diminished AvBD9 induction in chicken macrophages and jejunal explants in response to butyrate and FSK individually or in combination. Collectively, these results suggest the potential for concomitant use of butyrate and cAMP signaling activators in enhancing HDP expression, innate immunity, and disease resistance in both animals and humans.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular Immunology - Volume 57, Issue 2, February 2014, Pages 171-180
Journal: Molecular Immunology - Volume 57, Issue 2, February 2014, Pages 171-180
نویسندگان
Lakshmi T. Sunkara, Xiangfang Zeng, Amanda R. Curtis, Guolong Zhang,