کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5917442 1570734 2011 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Nucleosomes and C1q bound to glomerular endothelial cells serve as targets for autoantibodies and determine complement activation
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
Nucleosomes and C1q bound to glomerular endothelial cells serve as targets for autoantibodies and determine complement activation
چکیده انگلیسی

Various studies indicate a role for both anti-nucleosome and anti-C1q autoantibodies in glomerulonephritis in patients with systemic lupus erythematosus. However, a causal relationship between these autoantibodies and the development of lupus nephritis has not been fully established. Since injury of the endothelium is a major target in lupus nephritis we assessed the interaction of C1q and nucleosomes with glomerular endothelial cells in vitro in the presence or absence of autoantibodies against these antigens. We demonstrate a direct and dose-dependent binding of both nucleosomes and C1q to immortalized human glomerular endothelial cells (GEnC) in vitro, which in part is mediated by cell surface heparan sulfate. We demonstrate that nucleosomes and C1q serve as targets for monoclonal and polyclonal antibodies as well as for anti-nuclear autoantibodies from patients with systemic lupus erythematosus. An additive effect of anti-C1q autoantibodies on anti-nucleosome mediated complement activation was observed. Furthermore, we showed that the activation of complement on glomerular endothelial cells is mediated by the classical pathway since the deposition of C3 on GEnC is abrogated by MgEGTA and does not occur in C1q-depleted serum. Taken together, our studies demonstrate a direct binding of both nucleosomes and C1q to glomerular endothelial cells in vitro. The subsequent binding of autoantibodies against nucleosomes in patients with systemic lupus erythematosus is potentially pathogenic and autoantibodies against C1q seem to have an additional effect.

► Assessed the interaction of C1q and nucleosomes with glomerular endothelial cells in vitro in the presence or absence of autoantibodies. ► Direct binding of both nucleosomes and C1q to glomerular endothelial cells in vitro. ► Nucleosomes and C1q serve as targets for monoclonal and polyclonal antibodies from SLE patients. ► Additive effect of anti-C1q autoantibodies on anti-nucleosome mediated complement activation observed.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular Immunology - Volume 49, Issues 1–2, October–November 2011, Pages 75-83
نویسندگان
, , , , , , , , , ,