کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5918219 | 1163835 | 2010 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Stylicins, a new family of antimicrobial peptides from the Pacific blue shrimp Litopenaeus stylirostris
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Stylicins, a new family of antimicrobial peptides from the Pacific blue shrimp Litopenaeus stylirostris Stylicins, a new family of antimicrobial peptides from the Pacific blue shrimp Litopenaeus stylirostris](/preview/png/5918219.png)
چکیده انگلیسی
The present study reports the characterization of Ls-Stylicin1, a novel antimicrobial peptide from the penaeid shrimp, Litopenaeus stylirostris. The predicted mature peptide of 82 residues is negatively charged (theoretical pI = 5.0) and characterized by a proline-rich N-terminal region and a C-terminal region containing 13 cysteine residues. The recombinant Ls-Stylicin1 has been isolated in both monomeric and dimeric forms. Both display strong antifungal activity against Fusarium oxysporum (1.25 μM < MIC < 2.5 μM), a pathogenic fungus of shrimp, but lower antimicrobial activity against Gram (â) bacteria, Vibrio sp. (40 μM < MIC < 80 μM). However, rLs-Stylicin1 is able to agglutinate Vibrio penaeicidae in vitro in agreement with its potent LPS-binding activity on immobilized LPS of V. penaeicidae (dissociation constant (Kd) of 9.6 Ã 10â8 M). This molecule with no evident homology to other hitherto described antimicrobial peptides but identified herein several species of penaeid shrimp is thought to be the first member of a shrimp antimicrobial peptide family, which we termed stylicins.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular Immunology - Volume 47, Issue 6, March 2010, Pages 1269-1277
Journal: Molecular Immunology - Volume 47, Issue 6, March 2010, Pages 1269-1277
نویسندگان
J.L. Rolland, M. Abdelouahab, J. Dupont, F. Lefevre, E. Bachère, B. Romestand,