Estimating divergence dates and evaluating dating methods using phylogenomic and mitochondrial data in squamate reptiles

► A genomic dataset of 25 nuclear loci is generated for squamate reptiles. ► Phylogeny is estimated using parsimony, maximum likelihood, and Bayesian methods. ► Dates of divergence are estimated with fossil calibrations using r8s and BEAST. ► The same methods are compared with mitochondrial ND2 data. ► The dating methods are evaluated using subsamples of the 25 nuclear loci.