Phylogeny and species boundaries of the endemic species complex, Eliurus antsingy and E. carletoni (Rodentia: Muroidea: Nesomyidae), in Madagascar using mitochondrial and nuclear DNA sequence data

Current understanding of the taxonomy and species relationships within the genus Eliurus, a member of the endemic Malagasy rodent subfamily Nesomyinae, is largely based on morphological characters. Little has been published about the historical biogeography and diversification factors in members of this genus. Here we examined, using molecular data, the evolutionary relationships, and species boundaries within a species complex of Eliurus and the role of rivers in their evolution. Specifically, we tested the hypothesized monophyly of E. antsingy (west) and the recently described E. carletoni (north) using two mitochondrial genes, cytochrome b, the control region, and a nuclear marker, the Interstitial Retinol Binding Protein (IRBP). We also assessed the species boundaries of E. carletoni and examined phylogeographic patterns using two mitochondrial markers and an extensive geographic sampling. Molecular data show that members of the E. antsingy and E. carletoni complex together form a monophyletic group. Although samples provisionally assigned to E. carletoni based on morphological characters from different sites in northern Madagascar cluster in three separate phylogroups, they are monophyletic and assignable to this species. Rivers do not act as strict barriers to dispersal in E. carletoni. The demonstrated monophyly of both E. antsingy and E. carletoni contributes to the amounting evidence for a biogeographic link between the north and western regions of the island.