Physalin B inhibits Trypanosoma cruzi infection in the gut of Rhodnius prolixus by affecting the immune system and microbiota

► The natural compound, physalin B, inhibited parasite development in insect vector. ► Physalin B treated insects presented higher microbiota in the gut. ► Physalin B (topic and contact) diminished antibacterial activity in infected insects. ► Physalin B (oral) produced higher levels of nitrite and nitrate in infected insects.