Midgut proteins released by microapocrine secretion in Spodoptera frugiperda

► Double membrane (microapocrine) vesicles bud from lepidopteran midgut microvilli. ► Antibodies against microapocrine vesicles were prepared. ► Antibodies were used to screen a midgut expression cDNA library. ► Sequences were extended with midgut mRNA pyrosequencing data and annotated. ► Proteins delivered by microapocrine secretion were identified.