Ionic Liquid Effects on the Activity of β-Glycosidase for the Synthesis of Salidroside in Co-solvent Systems

The preparation of salidroside was successfully carried out in fourteen ionic liquids (ILs)-containing systems using β-glycosidase from black plum seeds for the first time. The optimum conditions were determined for C6MIm·BF4, pH, phosphate buffer content, and molar ratio of tyrosol to D-glucose to be 1% (v/v), 5.9, 20% (v/v), and 8:1, under which the initial reaction rate and yield were 3.3 mmol/(L·h) and 24.5%, respectively. Moreover, the effects of 1-alkylimidazolium-based ILs possessing different alkyl chain lengths from C2 to C10 and a variety of anions including BF4−, PF6−, Cl−, Br−, and I− on enzyme activity in co-solvent systems were investigated. The results indicate that the optimal chain length of the alkyl substituent on the imidazolium ring of the cation was C6.

摘要研究了混合溶剂体系中阴离子分别为 BF4−, PF6−, Cl−, Br−和 I−的 14 种离子液体对来源于黑布林糖苷酶催化合成红景天苷反应的影响. 结果表明, 在最佳反应条件下, β-糖苷酶的反应初速度和红景天苷的产率分别为 3.3 mmol/(L·h) 和 24.5%. 离子液体咪唑阳离子的烷基链长 (C2∼C10) 对 β-糖苷酶的活性影响较大, 当烷基链长为 C6 时, 糖苷酶表现出较高的催化活性.

The effects of 1-alkylimidazolium-based ionic liquids possessing different alkyl chain lengths (C2-C10) and various anions (BF4−, PF6−, Cl−, Br−, and I−) on β-glycosidase activity for the synthesis of salidroside were investigated.Figure optionsDownload as PowerPoint slide