Simultaneous measurement of endogenous steroid hormones and their metabolites with LC-MS/MS in faeces of a New World primate species, Cebus capucinus

► LC-MS/MS method to simultaneously measure HPA and HPG axes steroids in primate faeces. ► Physiologically induced variation in faecal steroid levels of capuchins was verified. ► Quantification of steroid hormones and their main metabolites in faeces of capuchins. ► Exploration of conjugation patterns of steroids in faeces of capuchins.