کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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5963439 | 1576128 | 2016 | 7 صفحه PDF | دانلود رایگان |
BackgroundPercutaneous coronary intervention is widely used for the treatment of coronary artery disease; however, significant challenges such as restenosis remain. Key to solving these problems is to inhibit smooth muscle cell activation while enhancing re-endothelialization. Early growth response-1 (Egr-1) is a transcription factor that regulates vascular smooth muscle cell (SMC) proliferation and migration through its control of an array of downstream genes.MethodsA “cocktail” of vascular endothelial growth factor (VEGF)-A, VEGF-D and cyclic RGD was tested for its ability to inhibit neointima formation and accelerate re-endothelialization following balloon injury to carotid arteries of rats.ResultsIn vitro, the cocktail stimulated endothelial cell growth yet inhibited smooth muscle cell growth. In vivo, cocktail-treated injured arteries exhibited reduced intimal thickening by >Â 50% (PÂ <Â 0.05). It increased both re-endothelialization and endothelial nitric oxide synthase (NOS) expression. Cocktail reduced Egr-1 expression, an effect blocked by the NOS inhibitor L-NG-nitroarginine methyl ester (L-NAME) that also prevented cocktail inhibition of neointima inhibition.ConclusionsThis combination may potentially be useful for the treatment of restenosis with concomitant stimulation of revascularization.
Journal: International Journal of Cardiology - Volume 220, 1 October 2016, Pages 185-191