کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5971513 1576184 2014 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Protective effect of salidroside on cardiac apoptosis in mice with chronic intermittent hypoxia
ترجمه فارسی عنوان
اثر محافظتی سالیدروزید بر آپوپتوز قلب در موشهای مبتلا به هیپوکسی متناوب مزمن
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی کاردیولوژی و پزشکی قلب و عروق
چکیده انگلیسی

BackgroundThe goal of this study is to determine if salidroside has protective effects on hypoxia-induced cardiac widely dispersed apoptosis in mice with severe sleep apnea model.MethodsSixty-four C57BL/6 J mice 5-6 months of age were divided into four groups, i.e. Control group (21% O2, 24 h per day, 8 weeks, n = 16); Hypoxia group (Hypoxia: 7% O2 60 s, 20% O2 alternating 60 s, 8 h per day, 8 weeks, n = 16); and Hypoxia + S10 and Hypoxia + S30 groups (Hypoxia for 1st 4 weeks, hypoxia pretreated 10 mg/kg and 30 mg/kg salidroside by oral gavage per day for 2nd 4 weeks, n = 16 and 16). The excised hearts from four groups were measured by the heart weight index, H&E staining, TUNEL-positive assays and Western blotting.ResultsTUNEL-positive apoptotic cells in mice heart were less in Hypoxia + S10 and Hypoxia + S30 than those in the Hypoxia group. Compared with Hypoxia, the protein levels of Fas ligand, Fas death receptors, Fas-Associated Death Domain (FADD), activated caspase 8, and activated caspase 3 (Fas pathways) were decreased in Hypoxia + S10 and Hypoxia + S30. In the mitochondria pathway, the protein levels of BcLx, Bcl2, and Bid (anti-apoptotic Bcl2 family) in Hypoxia + S10 and Hypoxia + S30 were more than those in Hypoxia. The protein levels of Bax, t-Bid, activated caspase 9, and activated caspase 3 were less in Hypoxia + S10 and Hypoxia + S30 than those in hypoxia.ConclusionsOur findings suggest that salidroside has protective effects on chronic intermittent hypoxia-induced Fas-dependent and mitochondria-dependent apoptotic pathways in mice hearts.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Cardiology - Volume 174, Issue 3, 1 July 2014, Pages 565-573
نویسندگان
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