کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5977 452 2013 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Spheroid formation of human thyroid cancer cells in an automated culturing system during the Shenzhou-8 Space mission
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Spheroid formation of human thyroid cancer cells in an automated culturing system during the Shenzhou-8 Space mission
چکیده انگلیسی

Human follicular thyroid cancer cells were cultured in Space to investigate the impact of microgravity on 3D growth. For this purpose, we designed and constructed a cell container that can endure enhanced physical forces, is connected to fluid storage chambers, performs media changes and cell harvesting automatically and supports cell viability. The container consists of a cell suspension chamber, two reserve tanks for medium and fixative and a pump for fluid exchange. The selected materials proved durable, non-cytotoxic, and did not inactivate RNAlater. This container was operated automatically during the unmanned Shenzhou-8 Space mission. FTC-133 human follicular thyroid cancer cells were cultured in Space for 10 days. Culture medium was exchanged after 5 days in Space and the cells were fixed after 10 days. The experiment revealed a scaffold-free formation of extraordinary large three-dimensional aggregates by thyroid cancer cells with altered expression of EGF and CTGF genes under real microgravity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 34, Issue 31, October 2013, Pages 7694–7705
نویسندگان
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