Orientating lipase molecules through surface chemical control for enhanced activity: A QCM-D and ToF-SIMS investigation

• Lipase molecules were orientated through controlled adsorption kinetics.
• The catalytic domain was exposed on hydrophilic silica due to conformation changes.
• Hydrophobic surfaces inhibited the opening of lipase’s lid domain.
• Lipase activity correlated well with lipase orientation in engineered biomaterials.