PEGylated ofloxacin nanoparticles render strong antibacterial activity against many clinically important human pathogens

- PEGylated nanoparticles were efficiently up taken by bacteria.
- PEGylated ofloxacin nanoparticles showed high antibacterial activity against human pathogens.
- PEGylation significantly inhibited Bacillus subtilis from developing bacterial resistance against OFX.