کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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599330 | 1454274 | 2015 | 8 صفحه PDF | دانلود رایگان |
• BG-Zn scaffolds showed a controlled release of Zn ions.
• Significant increase of cell proliferation and ALP activity of hBMSCs was found in BG-5Zn group.
• BG-5Zn scaffolds showed a significantly better ostogenesis when implanted in rat calvarial defects.
The biomaterials with high osteogenic ability are being intensively investigated. In this study, we evaluated the bioactivity and osteogenesis of BG-Zn scaffolds in vitro and in vivo with a rodent calvarial defects model. Zinc containing borosilicate bioactive glass was prepared by doping glass with 1.5, 5 and 10 wt.% ZnO (denoted as BG-1.5Zn, BG-5Zn and BG-10Zn, respectively). When immersed in simulated body fluid, dopant ZnO retarded the degradation process, but did not affect the formation of hydroxyapatite (HA) after long-period soaking. BG-Zn scaffolds showed controlled release of Zn ions into the medium for over 8 weeks. Human bone marrow derived stem cells (hBMSCs) attached well on the BG-1.5Zn and BG-5Zn scaffolds, which exhibited no cytotoxicity to hBMSCs. In addition, the alkaline phosphatase activity of the hBMSCs increased with increasing dopant amount in the glass, while the BG-10Zn group showed over-dose of Zn. Furthermore, when implanted in rat calvarial defects for 8 weeks, the BG-5Zn scaffolds showed a significantly better capacity to regenerate bone tissue compared to the non-doping scaffolds. Generally, these results showed the BG-Zn scaffolds with high osteogenic capacity will be promising candidates using in bone tissue repair and regeneration.
Zn-doped borosilicate bioactive glass scaffolds with high osteogenic capacity represent a promising candidate for their use in bone tissue repair and regeneration.Figure optionsDownload as PowerPoint slide
Journal: Colloids and Surfaces B: Biointerfaces - Volume 130, 1 June 2015, Pages 149–156