کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
599339 | 1454274 | 2015 | 7 صفحه PDF | دانلود رایگان |
• Negatively charged nanocellulose enhanced the colloidal stability of HA nanoparticles.
• Well-developed porous structure of the biocomposites was observed.
• HA–TOBC–Gel composite showed differentiation without toxicity to cells.
• Mechanical properties of the HA–TOBC based composites were improved significantly.
Nanofibrous 2,2,6,6-tetramethylpiperidine-1-oxyl(TEMPO)-oxidized bacterial cellulose (TOBC) was used as a dispersant of hydroxyapatite (HA) nanoparticles in aqueous solution. The surfaces of TOBC nanofibers were negatively charged after the reaction with the TEMPO/NaBr/NaClO system at pH 10 and room temperature. HA nanoparticles were simply adsorbed on the TOBC nanofibers (HA–TOBC) and dispersed well in DI water. The well-dispersed HA–TOBC colloidal solution formed a hydrogel after the addition of gelatin, followed by crosslinking with glutaraldehyde (HA–TOBC–Gel). The chemical modification of the fiber surfaces and the colloidal stability of the dispersion solution confirmed TOBC as a promising HA dispersant. Both the Young's modulus and maximum tensile stress increased as the amount of gelatin increased due to the increased crosslinking of gelatin. In addition, the well-dispersed HA produced a denser scaffold structure resulting in the increase of the Young's modulus and maximum tensile stress. The well-developed porous structures of the HA–TOBC–Gel composites were incubated with Calvarial osteoblasts. The HA–TOBC–Gel significantly improved cell proliferation as well as cell differentiation confirming the material as a potential candidate for use in bone tissue engineering scaffolds.
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Journal: Colloids and Surfaces B: Biointerfaces - Volume 130, 1 June 2015, Pages 222–228