کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
599349 1454274 2015 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Self-assembled enzyme–inorganic hybrid nanoflowers and their application to enzyme purification
ترجمه فارسی عنوان
آنزیم های خودآزمون نانوفیلترهای هیبرید معدنی و کاربرد آنها در تصفیه آنزیم
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی شیمی کلوئیدی و سطحی
چکیده انگلیسی


• We have synthesized the organic–inorganic nanoflowers to realize further purification of the crude soybean peroxidase.
• Compared to the free crude SBP, the SBP embedded in hybrid naonflowers exhibited an enhanced enzymatic activity using guaiacol as substrate.
• The hybrid nanoflowers also exhibited excellent reusability and reproducibility.

We report a novel method to synthesize organic–inorganic nanoflowers for crude soybean peroxidase (SBP) purification. A hierarchical flower-like spherical structure with hundreds of nanopetals was self-assembled by using crude SBP as the organic component and Cu3(PO4)2·3H2O as the inorganic component. The structure of the hybrid nanoflowers was confirmed by Fourier-transform infrared spectroscopy, X-ray diffraction, and energy-dispersive X-ray spectroscopy, and the enzymatic activity of SBP embedded in the hybrid nanoflowers was evaluated using guaiacol as substrate. Compared with free crude SBP in solution, SBP embedded in hybrid nanoflowers exhibited enhanced enzymatic activity (∼446%). The hybrid nanoflowers also exhibited excellent reusability and reproducibility during cycle analysis. These results demonstrate that synthesis of hybrid nanoflowers is an effective enzyme purification strategy.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Colloids and Surfaces B: Biointerfaces - Volume 130, 1 June 2015, Pages 299–304
نویسندگان
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