کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
599387 1454270 2015 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Synthesis and characterization of dendro-PLGA nanoconjugate for protein stabilization
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی شیمی کلوئیدی و سطحی
پیش نمایش صفحه اول مقاله
Synthesis and characterization of dendro-PLGA nanoconjugate for protein stabilization
چکیده انگلیسی


• We engineered Dendro-PLGA nano-conjugate for protection of insulin from degradation.
• Dendro PLGA nanoconjugate not only stabilizes the insulin but also work as a sustained release reservoir for insulin. Dendro PLGA nanoconjugate reduces the frequency of dosage and side effect associate with denatured protein.

The present investigation was aimed to develop the dendro-PLGA nanoconjugate (DPNC) for protection of insulin from degradation as well as its sustained release from nano-formulation. DPNC formulation was synthesized by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) coupling reaction and therapeutic efficacy of encapsulated protein (insulin) was measured by Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), Fourier transform infrared spectroscopy (FTIR) and circular dichorism (CD) spectroscopy. We have also demonstrated the ability of DPNC formulation to prevent the native conformation of insulin within the system by comparing the amount of free protein with the protein extracted from this system. Stability study further revealed that as compared to free protein, DPNC formulation was more efficient to stabilize the protein. Additionally, in vivo data of protein loaded system in rats showed that DPNC formulation can able to maintain the native structure of insulin and hence retain therapeutic efficacy of protein. The novel dendro-PLGA nanoconjugate not only stabilize the insulin but also work as sustained release reservoir for insulin which reduces the frequency of dosage and side effect associate with denatured protein.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Colloids and Surfaces B: Biointerfaces - Volume 134, 1 October 2015, Pages 279–286
نویسندگان
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